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Analysis Fibre in Animal Feed

 Fibre for Animal Digestion


Analysis fibre applies 3 parameters such as solid-liquid extraction, delignification, and gravimetry. Solid-liquid extraction is carried out to extract oil from solid product. The criteria solvent is volatile solvent like n-heksana, acetone, or petroleum ether. Vaporized solvent will become condensate. It will react with solid product and dissolved organic agent inside sample. The extraction will do 6 hours. Extraction solid-liquid uses soxhlet apparatus.
The second step for analysis fibre is delignification. Sample is extracted with acid (H2SO4) and base solution (NaOH). Acid solution is extracted and neutralized with water. It also is conducted with base solution. Delignification with base solution and acid solution is done to separate plant carbohydrate into degistable and Indegistable fraction (Fahey Jr et al, 2019). According to Ibrahim and Menkovska (2022),  Indigestible fraction fermentable fibers is altered by enteric bacteria in the colon are converted into physiological active metabolites such as short chain fatty acids and organic acids. Degistable fraction is able to dissolved with water  


Reference

Fahey, Jr, Novotny, L, Layton, B, and Mertens, D, 2019, Critical Factors in Determining Fiber Content of Feeds and Foods and Their Ingredients, J.AOAC. Int., 102(1), 52-60
Ibrahim, O and Menkovska, M, 2022, Dietary Fibers-Classification, Properties, Analysis and Function: A Review, 



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